- Mean wheal diameter of local and imported grass and weed pollens [ Time Frame: 18 months ] [ Designated as safety issue: Yes ]
compare mean wheal diameter of skin prick test of different concentration among local, imported allergen extract and specific IgE levels of Bermuda grass, Johnson grass and careless weed
- sIgE levels of Bermuda grass,Johnson grass and careless weed [ Time Frame: 18 months ] [ Designated as safety issue: Yes ]
Measure level of sIgE of Bermuda grass,Johnson grass and careless weed and compare to SPT results
Skin prick test with local and imported Bermuda, Johnson and careless weed allergen extracts at concentration of 10,000, 3,000, 1,000 and 300 AU/mL and measure specific IgE levels
Skin prick test (SPT) and specific IgE (sIgE) are approved for evaluation of allergen sensitization. Local allergen extracts are cheaper and more available but need standardization. Pollen allergen extracts: Bermuda, Johnson and careless weed are locally prepared and compared mean wheal diameter of skin prick test to imported allergens and also to specific IgE.